Grazia Maria Borrelli
Name: Borrelli Grazia Maria
Actual position: Third-level researcher in Agricultural Genetics
Address: Via R. Consagro, 26 – 71122 Foggia, Italy
Phone: +39 742972
Fax: +39 713150
EDUCATION AND TRAINING
From 17/03/2008 to 12/04/2008 Researcher III level at the CRA-VIV of Pescia (PT).
- From April 2003 to March 2007: pHD in Sustainable Agro-Ecosystems. Thesis title: “Effect of salinity on yield and quality of production characters in old and new wheat genotypes: Physiological aspects and approaches in Metabolomics”.
From 05/11/2002 to 04/04/2006: Research Fellowship “Genetic improvement of durum wheat with innovative methodologies for the development of resistance to biotic and abiotic stresses” - AMIFRUGAM project “Innovative approaches to genetic improvement of cultivated wheats based on the use of relative germplasm and techniques of molecular analysis”, with regard to the research issue: “Qualitative and agronomic assessment of wheat lines bearing new genes for resistance to stress and quality”.
- From July 1990 to April 1992: Educational grant “Agronomic aspects of the use of Azospirillum”; National Biotechnology Plan (MAF project) “Development of advanced technologies applied to plants”.
- From September 1987 to September 1988: “Theoretical and practical training: clinical and microbiological analysis”, by the Laboratory Analysis II - OORR of Foggia.
- 24 June 1987: Degree in Biological Sciences at Pisa University, with final mark 110/110 cum laude. Thesis Title: “Study on the correlation between structure and function of xanthine oxidase”.
Agronomic aspects of the use of Azospirilli
Association between nitrogen fixing bacteria of the genus Azospirillum and durum wheat roots and relative effects on the yield.
In vitro culture in durum wheat
Influence of genotype and culture conditions on callus induction and plant regeneration; establishment of conditions for anther culture; production of friable lines to stabilize suspension cultures from which to obtain protoplasts; production of optimized cell culture system to be used as acceptor to transfer exogenous genes.
Genetic transformation of durum wheat
Development of the experimental conditions to genetically transform intact tissues of durum wheat by way electroporation, Agrobatterium, and biolistic method; study of the risks associated with the release into the environment of genetically modified wheat, in particular with regard to gene flow and effects on microorganisms in the rhizosphere (Notifications B/IT/99/31 and B/IT/99/33).
Genetic transformation with genes for antagonist peptides able to inhibit the immune response triggered by gliadins in celiac disease.
Improvement of tolerance to drought and cold in cultivated plants: analysis of the transcription factor “WRKY38” function and its possible uses through the production of durum wheat genetically modified plants.
Lipoxygenase (LOX) and semolina and pasta colour
Relationship between carotenoid pigment content and lipoxygenase activity in determining the colour of semolina and pasta in varieties of different genetic origin; biochemical characterization of LOX and its involvement in the aging processes; distribution into the kernel of different components involved in colour expression; genetic and environmental variability of these components; technological implications of LOX in durum wheat; effect of modifications in the pasta processing on the enzymatic activities of LOX and peroxidase and the final colour of the pasta.
Genetic improvement of cultivated wheats based on the use of relatives species and cytogenetic techniques for the development of resistance to biotic and abiotic stress
Agronomic and qualitative evaluation of new wheat lines carrying genes for resistance to stress and quality obtained by way cytogenetic techniques.
Transcriptional Profiling in durum wheat in abiotic stress conditions
Identification of genes differentially expressed in response to heat and drought stress and to their combination by AFLP and array approaches.